Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice.
Identifieur interne : 004789 ( Main/Exploration ); précédent : 004788; suivant : 004790Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice.
Auteurs : Huali Jin [République populaire de Chine] ; Chong Xiao ; Ze Chen ; Youmin Kang ; Yijie Ma ; Kaichun Zhu ; Qifa Xie ; Yixian Tu ; Yang Yu ; Bin WangSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2005.
Descripteurs français
- KwdFr :
- Activation des lymphocytes (), Activation des lymphocytes (génétique), Activation des lymphocytes (immunologie), Adjuvants immunologiques (métabolisme), Animaux, Cytokines (immunologie), Femelle, Lymphocytes auxiliaires Th1 (), Lymphocytes auxiliaires Th1 (immunologie), Lévamisole (administration et posologie), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (génétique), Syndrome respiratoire aigu sévère (immunologie), Thérapie génétique (), Traitement médicamenteux adjuvant (), Vaccins à ADN (administration et posologie), Vaccins à ADN (génétique).
- MESH :
- administration et posologie : Lévamisole, Vaccins à ADN.
- génétique : Activation des lymphocytes, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Vaccins à ADN.
- immunologie : Activation des lymphocytes, Cytokines, Lymphocytes auxiliaires Th1, Protéines nucléocapside, Syndrome respiratoire aigu sévère.
- métabolisme : Adjuvants immunologiques.
- Activation des lymphocytes, Animaux, Femelle, Lymphocytes auxiliaires Th1, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Thérapie génétique, Traitement médicamenteux adjuvant.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (metabolism), Animals, Chemotherapy, Adjuvant (methods), Cytokines (immunology), Female, Genetic Therapy (methods), Levamisole (administration & dosage), Lymphocyte Activation (drug effects), Lymphocyte Activation (genetics), Lymphocyte Activation (immunology), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Severe Acute Respiratory Syndrome (genetics), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Th1 Cells (drug effects), Th1 Cells (immunology), Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics).
- MESH :
- chemical , administration & dosage : Levamisole, Vaccines, DNA.
- chemical , genetics : Nucleocapsid Proteins, Vaccines, DNA.
- chemical , immunology : Cytokines, Nucleocapsid Proteins.
- chemical , metabolism : Adjuvants, Immunologic.
- drug effects : Lymphocyte Activation, Th1 Cells.
- genetics : Lymphocyte Activation, Severe Acute Respiratory Syndrome.
- immunology : Lymphocyte Activation, Severe Acute Respiratory Syndrome, Th1 Cells.
- methods : Chemotherapy, Adjuvant, Genetic Therapy.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Female, Mice, Mice, Inbred BALB C.
Abstract
Vaccination against the SARS-CoV infection is an attractive means to control the spread of viruses in public. In this study, we employed a DNA vaccine technology with the levamisole, our newly discovered chemical adjuvant, to generate Th1 type of response. To avoid the enhancement antibody issue, genes encoding the nucleocapsid, membrane, and envelope protein of SARS-CoV were cloned and their expressions in mammalian cells were determined. After the intramuscular introduction into animals, we observed that the constructs of the E, M, and N genes could induce high levels of specific antibodies, T cell proliferations, IFN-gamma, DTH responses, and in vivo cytotoxic T cells activities specifically against SARS-CoV antigens. The highest immune responses were generated by the construct encoding the nucleocapsid protein. The results suggest that the N, M, and E genes could be used as the targets to prevent SARS-CoV infection in the DNA vaccine development.
DOI: 10.1016/j.bbrc.2005.01.048
PubMed: 15707974
Affiliations:
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Le document en format XML
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Chen</name></author><author><name sortKey="Kang, Youmin" sort="Kang, Youmin" uniqKey="Kang Y" first="Youmin" last="Kang">Youmin Kang</name></author><author><name sortKey="Ma, Yijie" sort="Ma, Yijie" uniqKey="Ma Y" first="Yijie" last="Ma">Yijie Ma</name></author><author><name sortKey="Zhu, Kaichun" sort="Zhu, Kaichun" uniqKey="Zhu K" first="Kaichun" last="Zhu">Kaichun Zhu</name></author><author><name sortKey="Xie, Qifa" sort="Xie, Qifa" uniqKey="Xie Q" first="Qifa" last="Xie">Qifa Xie</name></author><author><name sortKey="Tu, Yixian" sort="Tu, Yixian" uniqKey="Tu Y" first="Yixian" last="Tu">Yixian Tu</name></author><author><name sortKey="Yu, Yang" sort="Yu, Yang" uniqKey="Yu Y" first="Yang" last="Yu">Yang Yu</name></author><author><name sortKey="Wang, Bin" sort="Wang, Bin" uniqKey="Wang B" first="Bin" last="Wang">Bin Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15707974</idno><idno 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first="Youmin" last="Kang">Youmin Kang</name></author><author><name sortKey="Ma, Yijie" sort="Ma, Yijie" uniqKey="Ma Y" first="Yijie" last="Ma">Yijie Ma</name></author><author><name sortKey="Zhu, Kaichun" sort="Zhu, Kaichun" uniqKey="Zhu K" first="Kaichun" last="Zhu">Kaichun Zhu</name></author><author><name sortKey="Xie, Qifa" sort="Xie, Qifa" uniqKey="Xie Q" first="Qifa" last="Xie">Qifa Xie</name></author><author><name sortKey="Tu, Yixian" sort="Tu, Yixian" uniqKey="Tu Y" first="Yixian" last="Tu">Yixian Tu</name></author><author><name sortKey="Yu, Yang" sort="Yu, Yang" uniqKey="Yu Y" first="Yang" last="Yu">Yang Yu</name></author><author><name sortKey="Wang, Bin" sort="Wang, Bin" uniqKey="Wang B" first="Bin" last="Wang">Bin Wang</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2005" 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dosage)</term><term>Vaccines, DNA (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes ()</term><term>Activation des lymphocytes (génétique)</term><term>Activation des lymphocytes (immunologie)</term><term>Adjuvants immunologiques (métabolisme)</term><term>Animaux</term><term>Cytokines (immunologie)</term><term>Femelle</term><term>Lymphocytes auxiliaires Th1 ()</term><term>Lymphocytes auxiliaires Th1 (immunologie)</term><term>Lévamisole (administration et posologie)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (génétique)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Thérapie génétique ()</term><term>Traitement médicamenteux adjuvant ()</term><term>Vaccins à ADN (administration et posologie)</term><term>Vaccins à ADN (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Levamisole</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Cytokines</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adjuvants, Immunologic</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Lévamisole</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lymphocyte Activation</term><term>Th1 Cells</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lymphocyte Activation</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Activation des lymphocytes</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Activation des lymphocytes</term><term>Cytokines</term><term>Lymphocytes auxiliaires Th1</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Lymphocyte Activation</term><term>Severe Acute Respiratory Syndrome</term><term>Th1 Cells</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chemotherapy, Adjuvant</term><term>Genetic Therapy</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adjuvants immunologiques</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Femelle</term><term>Lymphocytes auxiliaires Th1</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère</term><term>Thérapie génétique</term><term>Traitement médicamenteux adjuvant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vaccination against the SARS-CoV infection is an attractive means to control the spread of viruses in public. In this study, we employed a DNA vaccine technology with the levamisole, our newly discovered chemical adjuvant, to generate Th1 type of response. To avoid the enhancement antibody issue, genes encoding the nucleocapsid, membrane, and envelope protein of SARS-CoV were cloned and their expressions in mammalian cells were determined. After the intramuscular introduction into animals, we observed that the constructs of the E, M, and N genes could induce high levels of specific antibodies, T cell proliferations, IFN-gamma, DTH responses, and in vivo cytotoxic T cells activities specifically against SARS-CoV antigens. The highest immune responses were generated by the construct encoding the nucleocapsid protein. The results suggest that the N, M, and E genes could be used as the targets to prevent SARS-CoV infection in the DNA vaccine development.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Kang, Youmin" sort="Kang, Youmin" uniqKey="Kang Y" first="Youmin" last="Kang">Youmin Kang</name><name sortKey="Ma, Yijie" sort="Ma, Yijie" uniqKey="Ma Y" first="Yijie" last="Ma">Yijie Ma</name><name sortKey="Tu, Yixian" sort="Tu, Yixian" uniqKey="Tu Y" first="Yixian" last="Tu">Yixian Tu</name><name sortKey="Wang, Bin" sort="Wang, Bin" uniqKey="Wang B" first="Bin" last="Wang">Bin Wang</name><name sortKey="Xiao, Chong" sort="Xiao, Chong" uniqKey="Xiao C" first="Chong" last="Xiao">Chong Xiao</name><name sortKey="Xie, Qifa" sort="Xie, Qifa" uniqKey="Xie Q" first="Qifa" last="Xie">Qifa Xie</name><name sortKey="Yu, Yang" sort="Yu, Yang" uniqKey="Yu Y" first="Yang" last="Yu">Yang Yu</name><name sortKey="Zhu, Kaichun" sort="Zhu, Kaichun" uniqKey="Zhu K" first="Kaichun" last="Zhu">Kaichun Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Jin, Huali" sort="Jin, Huali" uniqKey="Jin H" first="Huali" last="Jin">Huali Jin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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